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Image Search Results


ZKSCAN3 overexpression alleviates autophagy-related changes in mice. (A) Representative immunoblot images of P62, Beclin-1, LC3 I and LC3 II in the lung tissue following the indicated treatments. (B-E) Quantification of the pixel intensities reflected the relative levels of P62, Beclin-1 and LC3 II to GAPDH and LC3 II/ LC3 I. (F) The pathological changes and autophagosomes of the lung tissue in each group of mice by transmission electron microscopy (scale bar =1 μm). (G) TUNEL staining of lung tissues following the indicated treatments (scale bar=50 μm). Data are presented as mean ± SD (n=6 per group). One-way ANOVA followed by Tukey’s post-hoc test. **, P<0.01 vs . control group; ## , P<0.01 vs . LPS group. ANOVA, analysis of variance; DAPI, 4',6-diamidino-2-phenylindole; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; LPS, lipopolysaccharide; NC, negative control; SD, standard deviation; TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling.

Journal: Journal of Thoracic Disease

Article Title: ZKSCAN3 mitigates lipopolysaccharide-induced acute lung injury by restraining excessive autophagy via activation of the mTOR pathway

doi: 10.21037/jtd-2026-1611

Figure Lengend Snippet: ZKSCAN3 overexpression alleviates autophagy-related changes in mice. (A) Representative immunoblot images of P62, Beclin-1, LC3 I and LC3 II in the lung tissue following the indicated treatments. (B-E) Quantification of the pixel intensities reflected the relative levels of P62, Beclin-1 and LC3 II to GAPDH and LC3 II/ LC3 I. (F) The pathological changes and autophagosomes of the lung tissue in each group of mice by transmission electron microscopy (scale bar =1 μm). (G) TUNEL staining of lung tissues following the indicated treatments (scale bar=50 μm). Data are presented as mean ± SD (n=6 per group). One-way ANOVA followed by Tukey’s post-hoc test. **, P<0.01 vs . control group; ## , P<0.01 vs . LPS group. ANOVA, analysis of variance; DAPI, 4',6-diamidino-2-phenylindole; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; LPS, lipopolysaccharide; NC, negative control; SD, standard deviation; TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling.

Article Snippet: The antibodies utilized in the Western blot analysis included p-mTOR (#5536; Danvers, USA, CST), mTOR (#2983, CST), Beclin-1 (#3495, CST), LC3 (#4108, CST), p62 (#ab109012, Abcam; Cambridge, UK), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (#ab181602, Abcam), ZKSCAN3 (#S-C806828, LSBio; Seattle, USA), HRP-Goat anti Rabbit (#AS1107, ASPEN; Wuhan, China), HRP-Goat anti Mouse (#AS1106, ASPEN), HRP-Rabbit anti Goat (#AS1108, ASPEN), HRP-Goat anti Rat (#AS1093, ASPEN), and HRP-Rabbit anti Sheep (#AS1245, ASPEN).

Techniques: Over Expression, Western Blot, Tissue, Transmission Assay, Electron Microscopy, TUNEL Assay, Staining, Control, Analysis, Negative Control, Standard Deviation, End Labeling

ZKSCAN3 overexpression reduces autophagy-related changes in vitro . (A) Representative immunoblot images of P62, Beclin-1, LC3 I and LC3 II in lung epithelial cells A549 following the indicated treatments. (B-E) Quantification of the pixel intensities reflected the relative levels of P62, Beclin-1 and LC3 II to GAPDH and LC3 II/LC3 I. (F) Flow cytometry dot plots of lung epithelial cells A549 following the indicated treatments. (G) Apoptotic rate of lung epithelial cells A549. Data are presented as mean ± SD (n=6 per group). One-way ANOVA followed by Tukey’s post-hoc test. **, P<0.01 vs . control group; # , P<0.05 vs . LPS group; ## , P<0.01 vs . LPS group. ANOVA, analysis of variance; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; LPS, lipopolysaccharide; NC, negative control; SD, standard deviation.

Journal: Journal of Thoracic Disease

Article Title: ZKSCAN3 mitigates lipopolysaccharide-induced acute lung injury by restraining excessive autophagy via activation of the mTOR pathway

doi: 10.21037/jtd-2026-1611

Figure Lengend Snippet: ZKSCAN3 overexpression reduces autophagy-related changes in vitro . (A) Representative immunoblot images of P62, Beclin-1, LC3 I and LC3 II in lung epithelial cells A549 following the indicated treatments. (B-E) Quantification of the pixel intensities reflected the relative levels of P62, Beclin-1 and LC3 II to GAPDH and LC3 II/LC3 I. (F) Flow cytometry dot plots of lung epithelial cells A549 following the indicated treatments. (G) Apoptotic rate of lung epithelial cells A549. Data are presented as mean ± SD (n=6 per group). One-way ANOVA followed by Tukey’s post-hoc test. **, P<0.01 vs . control group; # , P<0.05 vs . LPS group; ## , P<0.01 vs . LPS group. ANOVA, analysis of variance; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; LPS, lipopolysaccharide; NC, negative control; SD, standard deviation.

Article Snippet: The antibodies utilized in the Western blot analysis included p-mTOR (#5536; Danvers, USA, CST), mTOR (#2983, CST), Beclin-1 (#3495, CST), LC3 (#4108, CST), p62 (#ab109012, Abcam; Cambridge, UK), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (#ab181602, Abcam), ZKSCAN3 (#S-C806828, LSBio; Seattle, USA), HRP-Goat anti Rabbit (#AS1107, ASPEN; Wuhan, China), HRP-Goat anti Mouse (#AS1106, ASPEN), HRP-Rabbit anti Goat (#AS1108, ASPEN), HRP-Goat anti Rat (#AS1093, ASPEN), and HRP-Rabbit anti Sheep (#AS1245, ASPEN).

Techniques: Over Expression, In Vitro, Western Blot, Flow Cytometry, Control, Analysis, Negative Control, Standard Deviation